Composite

Part:BBa_M36522:Design

Designed by: Scanner Cheung, Emily Marx   Group: Stanford BIOE44 - S11   (2013-10-29)


ALDH2 actuator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1495
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1602
    Illegal PstI site found at 1495
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1247
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1495
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1495
    Illegal NgoMIV site found at 305
    Illegal NgoMIV site found at 448
    Illegal NgoMIV site found at 961
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 367
    Illegal SapI.rc site found at 864


Design Notes

made sure ATG was removed from BCD since it was already present in GOI


Source

See subparts; BCD from Mutalik, GOI from Vega, terminator from registry

References